Rpb1
+Domains+and+domainlike+regions+of+Rpb1.+The+amino+acid+residue+numbers+at+the+domain+boundaries+are+indicated.+(B)+Ribbon+diagrams%2C+showing+the+location+of+Rpb1+within+Pol+II+[+front+and+top+views+of+the+enzyme%2C+as+previously+designated+(9)]%2C+and+Rpb1+alone.+Locations+of+NH2-+and+COOH-termini+are+indicated.+Color-coding+as+in+(A).+(C)+Secondary+structure+and+amino+acid+sequence+alignment.+Yeast+amino+acid+residue+numbers+are+indicated+above+the+sequence.+Secondary+structure+elements+were+identified+by+inspection+and+are+indicated+and+numbered+above+the+sequence+(boxes+for+helices%2C+arrows+for+strands).+Solid%2C+dotted%2C+and+dashed+lines+above+the+sequences+indicate+ordered%2C+partially+ordered%2C+and+disordered+loops%2C+respectively.+Alignment+of+Rpb1+from+yeast+(y)+with+human+Rpb1+(h)+and+E.+coli+subunit+(e)+was+initially+carried+out+with+CLUSTALW+(82)+and+then+edited+by+hand.+Alignment+of+the+E.+coli+sequence+is+based+on+the+structure+of+the+bacterial+enzyme+(30).+Regions+for+which+the+polypeptide+backbones+follow+the+same+course+are+indicated+by+gray+bars+below+the+sequences+(dotted+when+uncertain).+The+remaining+regions+could+not+be+aligned+because+of+disorder+or+because+they+differ+in+structure+so+that+alignment+is+meaningless.+Sequence+homology+blocks+A+to+H+(70)+are+indicated+below+the+sequences+by+black+bars.+Important+structural+elements+and+prominent+regions+involved+in+subunit+interactions+are+also+noted.+Residues+involved+in+Zn2%2B+and+Mg2%2B+coordination+are+highlighted+in+blue+and+pink%2C+respectively.+(D.jpg "Rpb1")
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