Cole1 Vector

Impact of targeted vector design on ColE1 plasmid replication Trends

The vector pHGF7604 The plasmid is bifunctional with the ColE1

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Schematic presentation of main expression vector library elements The

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ColE1 DNA plasmid cloning 60µg Nippon Gene Wako

ColE1 DNA plasmid cloning 60g Nippon Gene Wako

4 Schematic representation of ColE1 replication adapted from Tomizawa

The ColE1 Unidirectional Origin Acts as a Polar Replication Fork

Illustration of the mammalian expression vector constructs used in this

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A Graphical representation of pRB3 a cp26incompatible shuttle vector

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RNAI structure sequence comparison between common colE1 origins and

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4 Schematic representation of ColE1 replication adapted from Tomizawa

The pIMC Ecoli expression vectors The pIMC vectors have a ColE1

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HeLa cells were cotransfected with the neorbased L1 expression vector

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2 The mechanism of ColE1 plasmid replication by antisense RNA

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A mobilization region of ColE1 plasmid B Representation of the two

Resolution of ColE1 dimers requires a DNA sequence implicated in the

4 Schematic representation of ColE1 replication adapted from Tomizawa

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Physical maps of cosmid vectors prepared in sCos1 Evans et al 1989

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Figure 2 from Sitespecific mutagenesis in Legionella pneumophila by

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HfqAntisense RNA I Binding Regulates RNase EDependent RNA Stability

ColE1like plasmid copy number in tRNAoverproducing cells Copy number

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